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Kessler-GRM circle is intended for detection of bacteria of group of colibacillus on the basis of fermentation of lactose at sanitary inspection of foodstuff and objects of external environment (water, drains, etc.). Represents hydroscopic finely divided powder of gray-yellow color. STRUCTURE, G/L
Peptone 7,0 Pancreatic hydrolyzate of fish meal 3,0 Lactose 10,0 Bile the cleared dry 3,0 Crystal violet 0,04 Sodium carbonate 0,02±0,05 rn ready environment 7,5±0,2
- The preparation in number of 23,0 g is stirred in 1 l of distilled water, heated to boiling and boiled within 2-3 min.
- The hot environment is filtered via the wadded and gauze or paper filter and spill 5 ml in sterile test tubes with floats.
- Wednesday in test tubes will be sterilized autoclaving at temperature of 112 °C within 20 min.
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